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Gene Expression Data
Assay Details
Assay
Reference: J:16970 Chang CY, et al., Quantitative effects of the retinoblastoma gene on mouse development and tissue-specific tumorigenesis. Cell Growth Differ. 1993 Dec;4(12):1057-64
Assay type: Western blot
MGI Accession ID: MGI:2684767
Gene symbol: Rb1
Gene name: RB transcriptional corepressor 1
Antibody: anti-RB (clone G3-245)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Figures 3 and 4 in the paper seem to have been switched. The protein used in the western was obtained by using immunoprecipitation.
Results Image: 4B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
Lane 1 Transgenic sample: no data stored
Lane 2 Transgenic sample: no data stored
Lane 3 Transgenic sample: no data stored
Lane 4 Transgenic sample: no data stored
Lane 5 E13.0 (a) TS21: brain Absent involves: 129S7/SvEvBrd * C57BL/6 Rb1tm1Brd/Rb1tm1BrdNot Specified
Lane 6 E13.0 (a) TS21: brain Present (b) involves: 129S7/SvEvBrd * C57BL/6 Rb1tm1Brd/Rb1+Not Specified
Lane 7 E13.0 (a) TS21: brain Present Not Specified Not Specified
Lane 8 Transgenic sample: no data stored
Lane 9 Transgenic sample: no data stored
Notes:
(a) In the Materials and Methods the authors stated the embryos were E13 +/- 1 day. Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are 60% of those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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