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Gene Expression Data
Assay Details
Assay
Reference: J:43230 Kumar S, et al., Origin, expression and possible functions of the two alternatively spliced forms of the mouse Nedd2 mRNA. Cell Death Differ. 1997 JUL;4(5):378-387
Assay type: RT-PCR
MGI Accession ID: MGI:2684671
Gene symbol: Casp2
Gene name: caspase 2
Probe: Nedd2-pA, Nedd2-pB
Probe preparation: labelled with P32
Assay notes: Both transcripts were widely expressed, but the levels of each relative to one another varies. The RT-PCR products were blotted and then hybridized with transcript specific probes.
Results
Image: 3 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 404 bp (a) 343 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
MN2spl (25 pg) Control
Embryo day 10 E10.0 (c) TS16: embryo Present Not Specified 1 µg; poly-A+ RNA Not Specified Not Specified
Embryo day 18 E18.0 (c) TS26: embryo Present Not Specified 1 µg; poly-A+ RNA Not Specified Not Specified
Brain (3 weeks) P w 3 TS28: brain Present Not Specified 1 µg; poly-A+ RNA Not Specified Not Specified
Brain P adult TS28: brain Weak Weak 1 µg; poly-A+ RNA Not Specified Not Specified
Spleen P adult TS28: spleen Present Present 1 µg; poly-A+ RNA Not Specified Not Specified
Thymus P adult TS28: thymus Present Weak (d) 1 µg; poly-A+ RNA Not Specified Not Specified
Lung P adult TS28: lung Present Weak 1 µg; poly-A+ RNA Not Specified Not Specified
Gut P adult TS28: gut Present Not Specified 1 µg; poly-A+ RNA Not Specified Not Specified
Testis P adult TS28: testis Present Not Specified 1 µg; poly-A+ RNA Not Specified Male
Skeletal muscle P adult TS28: skeletal muscle Weak Weak 1 µg; poly-A+ RNA Not Specified Not Specified
Kidney P adult TS28: metanephros Present Not Specified 1 µg; poly-A+ RNA Not Specified Not Specified
MN2spl (100 pg) Control
MSN2.4 (100 pg) Control
Notes:
(a) This is the dominant transcript.
(b) This is a minor transcript.
(c) Age of embryo at noon of plug day not specified in reference.
(d) This was the tissue with the lowest level of expression of this transcript.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory