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Gene Expression Data
Assay Details
Assay
Reference: J:58474 Arango NA, et al., Targeted mutagenesis of the endogenous mouse Mis gene promoter: in vivo definition of genetic pathways of vertebrate sexual development. Cell. 1999 Nov 12;99(4):409-19
Assay type: RNase protection
MGI Accession ID: MGI:2684579
Gene symbol: Amh
Gene name: anti-Mullerian hormone
Probe: MIS probe
Probe preparation: Antisense RNA
Visualized with: Autoradiography
Results Image: 6A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E13.5 +/+ E13.5 (a) TS21: testis Present 2-3 µg; total RNA Not Specified Male
E13.5 R1/R1 E13.5 (a) TS21: testis Present (b) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2.1Bhr/Amhtm2.1BhrMale
E13.5 R1/R1-neo E13.5 (a) TS21: testis Present (c) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2Bhr/Amhtm2.1BhrMale
E15.5 +/+ E15.5 (a) TS23: testis Present 2-3 µg; total RNA Not Specified Male
E15.5 R1/R1 E15.5 (a) TS23: testis Present (b) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2.1Bhr/Amhtm2.1BhrMale
E15.5 R1/R1-neo E15.5 (a) TS23: testis Present (c) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2Bhr/Amhtm2.1BhrMale
9dpp +/+ P9 TS28: testis Present 2-3 µg; total RNA Not Specified Male
9dpp R1/R1 P9 TS28: testis Present (b) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2.1Bhr/Amhtm2.1BhrMale
9dpp R1/R1-neo P9 TS28: testis Present (c) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2Bhr/Amhtm2.1BhrMale
12dpp +/+ P12 TS28: testis Present 2-3 µg; total RNA Not Specified Male
12dpp R1/R1 P12 TS28: testis Absent (b) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2.1Bhr/Amhtm2.1BhrMale
12dpp R1/R1-neo P12 TS28: testis Not Specified (c) 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm2Bhr/Amhtm2.1BhrMale
loxP/loxP E15.5 E15.5 TS23: testis Present 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm5.1Bhr/Amhtm5.1BhrMale
loxP/loxP 12dpp P12 TS28: testis Present 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm5.1Bhr/Amhtm5.1BhrMale
loxP/loxP Liver P TS28: liver Absent 2-3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Amhtm5.1Bhr/Amhtm5.1BhrMale
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels in R1/R1 testes are 3 fold lower than in wild type.
(c) Expression levels in R1/R1-neo testes are slightly lower than those seen in R1/R1 testes (which are significantly lower than those seen in wild type testes).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory