Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:60639 Wang B, et al., Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb. Cell. 2000 Feb 18;100(4):423-34
Assay type:	Western blot
MGI Accession ID:	MGI:2684518
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:	Gli3ab1
Assay notes:	Expression levels assigned by curator using quantification data found in part E of the figure.

Results

Image: 7D

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	190 kDa	83 kDa	Genetic Background	Mutant Allele(s)	Sex	Note
Anterior	E10.5 (a)	TS17: limb	Weak (b)	Strong (c)	CD-1		Not Specified	anterior portion of the limb bud
Posterior	E10.5 (a)	TS17: limb	Weak (b)	Moderate	CD-1		Not Specified	posterior portion of the limb bud
Anterior	Control
Posterior	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The unprocessed form of the protein was found in relatively low levels throughout the limb; its abundance did not change between the anterior and posterior portions of the limb.
(c) The processed form of the protein is much more abundant in the anterior portion of the limb.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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