Plp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:87296 Ikenaka K, et al., Selective expression of DM-20, an alternatively spliced myelin proteolipid protein gene product, in developing nervous system and in nonglial cells. J Neurochem. 1992 Jun;58(6):2248-53
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684483
Gene symbol:	Plp1
Gene name:	proteolipid protein (myelin) 1

Probe:	PLP-PCR1, PLP-PCR2
Visualized with:	Autoradiography
Assay notes:	RT-PCR products were transferred to membranes sequentially hybridized with P32-labeled E4II (MGI:2684443), DM-20 (MGI:2684446) and PLP-ONLY (MGI:2684444) probes.

Results

Image: 3

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	1023 bp (a)	918 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
cDNA	Control
E11	E11.0 (c)	TS18: head	Absent	Present	2 µg; total RNA	ICR		Not Specified
E12	E12.0 (c)	TS20: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
E13	E13.0 (c)	TS21: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
E14	E14.0 (c)	TS22: brain	Absent	Present (d)	2 µg; total RNA	ICR		Not Specified
E15	E15.0 (c)	TS23: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
E16	E16.0 (c)	TS24: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
E17	E17.0 (c)	TS25: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
E18	E18.0 (c)	TS26: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
P2	P2	TS27: brain	Trace (e)	Present	2 µg; total RNA	ICR		Not Specified
P8	P8	TS28: brain	Present	Present	2 µg; total RNA	ICR		Not Specified
P18	P18	TS28: brain	Present	Present	2 µg; total RNA	ICR		Not Specified

Notes:
(a) PLP transcript shown in Fig. 3A and C; recognized by E4II and PLP-ONLY probes.
(b) DM-20 transcript shown in Fig. 3A and B; recognized by E4II and DM-20 probes.
(c) Age of embryo at noon of plug day not specified in reference.
(d) Level of DM-20 peaked at E14.
(e) Authors state that PLP became detectable in P2 brain on much longer exposure

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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