Plp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:87296 Ikenaka K, et al., Selective expression of DM-20, an alternatively spliced myelin proteolipid protein gene product, in developing nervous system and in nonglial cells. J Neurochem. 1992 Jun;58(6):2248-53
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684482
Gene symbol:	Plp1
Gene name:	proteolipid protein (myelin) 1

Probe:	PLP-PCR1, PLP-PCR2
Visualized with:	Autoradiography
Assay notes:	RT-PCR products were transferred to membranes sequentially hybridized with P32-labeled E4II (MGI:2684443), DM-20 (MGI:2684446) and PLP-ONLY (MGI:2684444) probes.

Results

Image: 2

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	1023 bp (a)	918 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
cDNA	Control
E16	E16.0 (c)	TS24: brain	Absent	Present	2 µg; total RNA	ICR		Not Specified
P8	P8	TS28: brain	Present	Present	2 µg; total RNA	ICR		Not Specified
P18	P18	TS28: brain	Present	Present	2 µg; total RNA	ICR		Not Specified
x1/10	Control
x1/100	Control
x1/1000	Control
Liver	P adult	TS28: liver	Absent	Absent	2 µg; total RNA	ICR		Not Specified
Sciatic nerve	Non-mouse sample: no data stored
NIH3T3	Cultured cells: no data stored
NIH/PLP	Cultured cells: no data stored
cDNA	Control

Notes:
(a) PLP transcript shown in Fig. 2A, B and D; recognized by E4II and PLP-ONLY probes.
(b) DM-20 transcript shown in Fig. 2A, B and C; recognized by E4II and DM-20 probes.
(c) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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