Plp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:18263 Kagawa T, et al., Fate of jimpy-type oligodendrocytes in jimpy heterozygote. J Neurochem. 1994 May;62(5):1887-93
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684450
Gene symbol:	Plp1
Gene name:	proteolipid protein (myelin) 1

Probe:	PLP-PCR1, PLP-PCR2
Visualized with:	Autoradiography
Assay notes:	RT-PCR products were transferred to membranes sequentially hybridized with P32-labeled E5II (MGI:2684447) and E5del (MGI:2684448) probes.

Results

Image: 4A

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (d)	Amount	Genetic Background	Mutant Allele(s)	Sex
P9 wild	P9	TS28: brain	Present	Absent	Present	Absent	Not Specified	Not Specified		Not Specified
P9 jp/X	P9	TS28: brain	Present	Present	Present	Present	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Plp1⁺	Female
P9 jp/Y	P9	TS28: brain	Absent	Present	Absent	Present	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Y	Male
P18 wild	P18	TS28: brain	Present	Absent	Present	Absent	Not Specified	Not Specified		Not Specified
P18 jp/X	P18	TS28: brain	Present	Weak	Present	Weak	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Plp1⁺	Female
P18 jp/Y	P18	TS28: brain	Absent	Present	Absent	Present	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Y	Male

Notes:
(a) Labeled PLP ; recognized by the E5II probe.
(b) Labeled DM20; recognized by the E5II probe
(c) Labeled jp-PLP; recognized by the E5del probe
(d) Labeled jp-DM20; recognized by the E5del probe

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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