Plp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:18263 Kagawa T, et al., Fate of jimpy-type oligodendrocytes in jimpy heterozygote. J Neurochem. 1994 May;62(5):1887-93
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684449
Gene symbol:	Plp1
Gene name:	proteolipid protein (myelin) 1

Probe:	PLP-PCR1, PLP-PCR2
Visualized with:	Autoradiography
Assay notes:	RT-PCR products were transferred to membranes sequentially hybridized with P32-labeled E4II (MGI:2684443), DM-20 (MGI:2684446) and E5del (MGI:2684448) probes.

Results

Image: 2

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	1023 bp (b)	949 bp (c)	918 bp (d)	844 bp (e)	Amount	Genetic Background	Mutant Allele(s)	Sex
E16 jp/Y	E16.0 (a)	TS24: brain	Absent	Present	Absent	Present	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Y	Male
E16 wild	E16.0 (a)	TS24: brain	Present	Absent	Present	Absent	Not Specified	Not Specified		Not Specified
P2 jp/Y	P2	TS27: brain	Absent	Present	Absent	Present	Not Specified	B6CB-A^w-J/A Eda^Ta Plp1^jp	Plp1^jp/Y	Male
P2 wild	P2	TS27: brain	Present	Absent	Present	Absent	Not Specified	Not Specified		Not Specified
cDNA	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Labeled normal PLP in Fig. 2A; recognized by the E4II probe.
(c) Labeled jp PLP in Figs. 2A and 2C; recognized by E4II and E5del probes.
(d) Labeled normal DM-20 in Figs. 2A and 2B; recognized by E4II and DM-20 probes.
(e) Labeled jp DM-20 in Figs. 2A, 2B and 2C; recognized by E4II, DM-20 and E5del probes.

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