Antibody:	anti-Lin28
Assay notes:	Specimens were formalin fixed.

Structure	Level	Pattern	Image	Note
TS9: embryo	Strong		1A
TS9: embryo ectoderm	Strong		1A
TS9: embryo endoderm	Strong		1A
TS9: extraembryonic ectoderm	Strong		1A
TS9: extraembryonic endoderm	Strong		1A

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1B	Expression is observed in all three germ layers.
TS11: extraembryonic ectoderm	Present		1B
TS11: extraembryonic endoderm	Present		1B
TS11: trophectoderm	Present	Single cells	1B	Expression in some trophoblast cells.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			The authors state that there is broad expression in a variety of tissues derived from the three germ layers and in extraembryonic tissues.
TS13: extraembryonic component	Present			The authors state that there is broad expression in a variety of tissues derived from the three germ layers and in extraembryonic tissues.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		2A

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch ectoderm	Present		2B	There is apical localization of the protein.

Structure	Level	Pattern	Image	Note
TS15: body cavity or lining	Present	Regionally restricted	2C	Expression is in the epithelia covering the coelomic cavity.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Strong		2D
TS15: endocardial lining	Absent		2D
TS15: intraembryonic coelom	Absent		2D

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2E

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Weak
TS15: yolk sac	Very strong
TS15: extraembryonic component	Very strong	Regionally restricted		Expression is in the parietal tissue.

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Present	Regionally restricted	3A	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present	Regionally restricted	3B	Expression is in some areas of the epithelium, absent in the more proximal regions.

Structure	Level	Pattern	Image	Note
TS17: lung epithelium	Present	Regionally restricted	3C

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Strong		4A

Structure	Level	Pattern	Image	Note
TS23: tail paraxial mesenchyme	Present	Regionally restricted	4B	Expression is in the myotome of the somites.

Structure	Level	Pattern	Image	Note
TS23: foregut	Present	Regionally restricted	4C	Expression is in the foregut epithelium.

Structure	Level	Pattern	Image	Note
TS23: stomach epithelium	Present		4D
TS23: stomach glandular region epithelium	Absent		4D

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present	Regionally restricted	4E	Expression is in some tubules.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted		More differentiated bronchi lost expression.
TS23: central nervous system	Weak

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Absent		5A

Structure	Level	Pattern	Image	Note
TS25: cardiac muscle tissue	Present		5B

Structure	Level	Pattern	Image	Note
TS25: skeletal muscle	Present		5C
TS25: skin	Present		5C

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	6A	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Present	Regionally restricted	6B	Expression is in the developing intralobar duct epithelium, absent in the main duct epithelia.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Regionally restricted	6C	Expression is in the epithelial cells of the loop of Henle and the collecting duct.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present		6D

Structure	Level	Pattern	Image	Note
TS28: lung	Absent
TS28: skeletal muscle	Present