Igll1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:67011 Bain G, et al., E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements. Cell. 1994 Dec 2;79(5):885-92
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684395
Gene symbol:	Igll1
Gene name:	immunoglobulin lambda-like polypeptide 1

Probe:	lambda5-pG, lambda5-pH
Assay notes:	The RT-PCR products were hybridized with a gene specific primer.

Results

Image: 5B lambda5

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
+/-	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3⁺	Not Specified
+/-	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3⁺	Not Specified
-/-	E15.5 (a)	TS23: liver	Present (b)	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3^tm1Cmu	Not Specified
-/-	E15.5 (a)	TS23: liver	Present (b)	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3^tm1Cmu	Not Specified
+/-	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3⁺	Not Specified
+/+	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	Not Specified		Not Specified
+/+	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	Not Specified		Not Specified
+/-	E15.5 (a)	TS23: liver	Present	3 µg; total RNA	either: 129P2/OlaHsd-Tcf3^tm1Cmu or (involves: 129P2/OlaHsd * FVB/N)	Tcf3^tm1Cmu/Tcf3⁺	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors stated that expression levels were dramatically reduced in the homozygotes relative to those seen in wild-type and heterozygotic embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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