Ghr Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:26722 Southard JN, et al., Growth hormone (GH) receptor and GH-binding protein messenger ribonucleic acids with alternative 5'-untranslated regions are differentially expressed in mouse liver and placenta. Endocrinology. 1995 Jul;136(7):2913-21
Assay type:	Northern blot
MGI Accession ID:	MGI:2684378
Gene symbol:	Ghr
Gene name:	growth hormone receptor

Probe:	E4IN probe
Probe preparation:	Antisense labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The identical hybridization pattern was observed in another assay (MGI:2684379). The ratio of the Ghr/Ghbp isoforms differed between the tissues. The probe used in this assay was 5' labelled.

Results

Image: 4_Exon 4

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	3.9 kb (a)	1.4 kb (b)	1.2 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
liver	P adult	TS28: liver	Present	Absent	Present	1 µg; poly-A+ RNA	Swiss Webster		Female	pooled from 3-5 dpc 16.5 pregnant mice
placenta	E16.5 (c)	TS28: placenta	Present	Present	Absent	10 µg; poly-A+ RNA	Swiss Webster		Female	pooled from 3-5 dpc 16.5 pregnant mice

Notes:
(a) This band represents the Ghr isoform and the band size varied from 3.9-4.2kb.
(b) This band represents the Ghbp isoform.
(c) Age normalized so that noon of plug day = E0.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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