Ghr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:26722 Southard JN, et al., Growth hormone (GH) receptor and GH-binding protein messenger ribonucleic acids with alternative 5'-untranslated regions are differentially expressed in mouse liver and placenta. Endocrinology. 1995 Jul;136(7):2913-21
Assay type:	RT-PCR
MGI Accession ID:	MGI:2684369
Gene symbol:	Ghr
Gene name:	growth hormone receptor

Probe:	E4IN
Assay notes:	The RT-PCR reaction used a gene-specific primer and an anchor primer. After electrophoresis, the gel was Southern blotted and hybridized with a random primed labeled probe (MGI:2684349) corresponding to the hormone-binding domain.

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	150 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Liver	P adult	TS28: liver	Present	2 µg; poly-A+ RNA	Swiss Webster		Female	dpc 16.5 pregnant mouse
Placenta	E16.5 (b)	TS28: placenta	Present	2 µg; poly-A+ RNA	Swiss Webster		Female

Notes:
(a) The band was actually a smear of several bands ranging in size from 150-550bp. The distribution of bands within this range was different for the two samples.
(b) Age normalized so that noon of plug day = E0.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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