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Gene Expression Data
Assay Details
Assay
Reference: J:31256 Gupta C, et al., Stimulation of epidermal growth factor gene expression during the fetal mouse reproductive tract differentiation: role of androgen and its receptor. Endocrinology. 1996 Feb;137(2):705-11
Assay type: RT-PCR
MGI Accession ID: MGI:2684303
Gene symbol: Egf
Gene name: epidermal growth factor
Probe: ME1, ME2
Probe preparation: labelled with P32
Visualized with: Autoradiography
Assay notes: This assay was a QCPCR (quantitative competitive PCR). The competing RNA was an in vitro transcribed Egf RNA containing a deletion with a known dose response curve. The ratios of densitometric signal intensities were plotted to determine relative concentration.
Results Image: 5A
 Sample Information Bands Other Sample Information
Lane AgeStructure 173 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
.4 E18.0 (a) TS26: male reproductive system Not Specified 0.4 µg; total RNA CD-1 Male pooled reproductive tracts
.2 E18.0 (a) TS26: male reproductive system Not Specified 0.2 µg; total RNA CD-1 Male pooled reproductive tracts
.1 E18.0 (a) TS26: male reproductive system Not Specified 0.1 µg; total RNA CD-1 Male pooled reproductive tracts
.05 E18.0 (a) TS26: male reproductive system Not Specified 0.05 µg; total RNA CD-1 Male pooled reproductive tracts
.025 E18.0 (a) TS26: male reproductive system Not Specified 0.025 µg; total RNA CD-1 Male pooled reproductive tracts
0 E18.0 (a) TS26: male reproductive system Not Specified 0 µg; total RNA CD-1 Male pooled reproductive tracts
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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