About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:87042 Krebs O, et al., Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud. Development. 2003 Dec;130(24):6037-47
Assay type: RT-PCR
MGI Accession ID: MGI:2684129
Gene symbol: Shh
Gene name: sonic hedgehog
Results
Image: 6A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
22 pUC standard Control
raz/raz FL E11.5 (a) TS19: forelimb bud Weak (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz HL E11.5 (a) TS19: hindlimb bud Weak (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz head E11.5 (a) TS19: head Present Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz body E11.5 (a) TS19: trunk Present Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
wt/raz FL E11.5 (a) TS19: forelimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz E11.5 (a) TS19: hindlimb bud Present (c) Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz head E11.5 (a) TS19: head Present Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
wt/raz body E11.5 (a) TS19: trunk Present Not Specified; total RNA C3.D2-Raz Raz/Raz+Not Specified
raz/raz FL E11.5 (a) TS19: forelimb bud Weak (b) Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz head E11.5 (a) TS19: head Present Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
raz/raz body E11.5 (a) TS19: trunk Present Not Specified; total RNA C3.D2-Raz Raz/RazNot Specified
wt/wt FL E11.5 (a) TS19: forelimb bud Present Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt HL E11.5 (a) TS19: hindlimb bud Present Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt head E11.5 (a) TS19: head Present Not Specified; total RNA involves: C3H/HeJ Not Specified
wt/wt body E11.5 (a) TS19: trunk Present Not Specified; total RNA involves: C3H/HeJ Not Specified
water control Control
water control Control
genomic DNA Control
genomic DNA Control
genomic DNA Control
Lane 22 Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report a definitive, low level of expression (10-20% of wt/wt), that was posteriorly restricted (data not shown).
(c) Authors state that expression was 40-80% that of wt/wt.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/28/2026
MGI 6.24 		The Jackson Laboratory