Ghr RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:22633 Ilkbahar YN, et al., Expression and distribution of messenger ribonucleic acids for growth hormone (GH) receptor and GH-binding protein in mice during pregnancy. Endocrinology. 1995 Feb;136(2):386-92
Assay type:	RNase protection
MGI Accession ID:	MGI:2684118
Gene symbol:	Ghr
Gene name:	growth hormone receptor

Probe:	GHBP probe1
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Phosphorimaging

Results

Image: 4A

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	256 bp (b)	150 bp (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
F14	E14.5 (a)	TS22: liver	Present	Present	Not Specified; total RNA	Swiss Webster		Pooled	7-10 pooled livers
F16	E16.5 (a)	TS24: liver	Present	Present	Not Specified; total RNA	Swiss Webster		Pooled	5 pooled livers
F18	E18.5 (a)	TS26: liver	Present	Present	Not Specified; total RNA	Swiss Webster		Pooled	5 pooled livers
6	P6	TS28: liver	Present	Present	Not Specified; total RNA	Swiss Webster		Pooled	5 pooled livers

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) This band represents the GHBP isoform. Expression level gradually increased with fetal age and peaked at P6 (2.5-fold higher than at E14.5).
(c) This band (doublet) represents the GHR isoform. Expression level gradually increased with fetal age and peaked at P6 (2.5-fold higher than at E14.5). The GHR isoform was more abundant than the GHBP isoform in all samples.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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