Ghr RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:22633 Ilkbahar YN, et al., Expression and distribution of messenger ribonucleic acids for growth hormone (GH) receptor and GH-binding protein in mice during pregnancy. Endocrinology. 1995 Feb;136(2):386-92
Assay type:	RNase protection
MGI Accession ID:	MGI:2684116
Gene symbol:	Ghr
Gene name:	growth hormone receptor

Probe:	GHBP probe1
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Phosphorimaging
Assay notes:	All of the pregnant or lactating mice had no fewer than 8 embryos or suckling pups. The tissue was inguinal mammary gland.

Results

Image: 2B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	256 bp (a)	150 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
NP	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	non-pregnant mouse.
8	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 8.5.
10	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 10.5.
12	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 12.5.
14	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 14.5.
16	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 16.5.
18	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 18.5.
L	P adult	TS28: mammary gland	Present	Present	10 µg; total RNA	Swiss Webster		Female	lactating mouse at postnatal day 6.

Notes:
(a) This band represents the GHBP isoform. Expression level started to decrease on E8.5 and by E16.5 was 4-fold lower than in the non-pregnant sample. The lactating sample was 7-fold lower than the non-pregnant sample.
(b) This band (doublet) represents the GHR isoform. Expression level started to decrease on E8.5 and by E16.5 was 4-fold lower than in the non-pregnant sample. The lactating sample was 7-fold lower than the non-pregnant sample.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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