Ghr RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:22633 Ilkbahar YN, et al., Expression and distribution of messenger ribonucleic acids for growth hormone (GH) receptor and GH-binding protein in mice during pregnancy. Endocrinology. 1995 Feb;136(2):386-92
Assay type:	RNase protection
MGI Accession ID:	MGI:2684115
Gene symbol:	Ghr
Gene name:	growth hormone receptor

Probe:	GHBP probe1
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Phosphorimaging
Assay notes:	All of the pregnant or lactating mice had no fewer than 8 embryos or suckling pups.

Results

Image: 2A

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	256 bp (a)	150 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
NP	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	non-pregnant mouse.
8	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 8.5.
10	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 10.5.
12	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 12.5.
14	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 14.5.
16	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 16.5.
18	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	pregnant mouse at dpc 18.5.
L	P adult	TS28: liver	Present	Present	5 µg; total RNA	Swiss Webster		Female	lactating mouse at postnatal day 6.

Notes:
(a) This band represents the GHBP isoform. Expression level started to increase on E10.5 and by E14.5-18.5 was 12-fold higher than in the non-pregnant sample. The non-pregnant and lactating samples had similar levels.
(b) This band (doublet) represents the GHR isoform. Expression level started to increase on E10.5 and reached a maximum at E14.5 of 6-fold higher than the non-pregnant sample and plateaued thereafter. The non-pregnant and lactating samples had similar levels.

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