Gene Expression Data
Assay Details
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Assay
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Reference:
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J:87042
Krebs O, et al., Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud. Development. 2003 Dec;130(24):6037-47
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2684105
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 probe5
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
8D:
embryonic day 10.5
(more  )
Note: Left and right forelimbs.
Specimen
8D:
(close  )
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Genetic Background:
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involves: C3H/HeJ
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left and right forelimbs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
8D
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Authors report that expression is progressively repressed beginning posteriorly and advancing anteriorly.
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Specimen
8E:
embryonic day 10.5; Raz/Raz +
(more )
Note: Left and right forelimbs.
Specimen
8E:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left and right forelimbs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
8E
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Authors state that the progressive repression is somewhat attenuated in heterozygotes.
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Specimen
8F:
embryonic day 10.5; Raz/ Raz
(more )
Note: Left and right forelimbs.
Specimen
8F:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left and right forelimbs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
8F
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Little or no regression of expression from the posterior border.
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Specimen
8G:
embryonic day 11.5
(more )
Note: Left forelimb.
Specimen
8G:
(close )
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Genetic Background:
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involves: C3H/HeJ
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
8G
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Authors report that expression is preogressively repressed beginning posteriorly and advancing anteriorly.
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Specimen
8H:
embryonic day 11.5; Raz/Raz +
(more )
Note: Left forelimb.
Specimen
8H:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
8H
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Authors state that the progressive repression is somewhat attenuated in heterozygotes.
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Specimen
8I:
embryonic day 11.5; Raz/ Raz
(more )
Note: Left forelimb.
Specimen
8I:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
8I
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Expression remains uniform throughout the anterior-posterior axis.
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Analysis Tools
