Antibody:	BEN antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present		1G	Staining is in the cytoplasm.
TS6: embryo	Absent		1G

Structure	Level	Pattern	Image	Note
TS8: embryo endoderm	Present		1I	Staining is in the cytoplasm.
TS8: extraembryonic endoderm	Present		1I	Staining is in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS9: embryo endoderm	Present		1J	Staining is in the cytoplasm. Higher magnification was shown in the inset.
TS9: extraembryonic endoderm	Present		1J	Staining is in the cytoplasm. Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present		1K	Staining is nuclear. Higher magnification was shown in the inset.
TS11: embryo mesoderm	Present		1K	Staining is nuclear. Higher magnification was shown in the inset.
TS11: neural ectoderm	Present		1K	Staining is nuclear. Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Very strong		1L
TS13: future prosencephalon	Strong		1L
TS13: head mesenchyme	Strong		1L
TS13: cardiac muscle tissue	Strong		1L	Expression is present in the endocardium and myocardium.
TS13: heart	Strong		1L	Expression is present in the endocardium and myocardium.
TS13: foregut diverticulum	Strong		1L
TS13: paraxial mesenchyme	Strong		1L
TS13: somite	Strong		1L
TS13: extraembryonic component	Present	Regionally restricted	1L	Staining is in the amnion and allantois.
TS13: primitive streak	Present		1L
TS13: lateral plate mesoderm	Present		1L
TS13: trunk unsegmented mesenchyme	Absent		1L

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Regionally restricted	2C	Expression is in post-mitotic neurons in the ventricular zone, intermediate zone and cortical plate, but not in the subventricular zone. Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS27: brain	Present		2D

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		2E
TS28: pons	Present		2E

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	3B	Expression is in the dorsoventral region of the developing neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	3C	Expression is in the ventrolateral region from the cervical to lumbar regions.
TS15: neural tube floor plate	Present		3C

Structure	Level	Pattern	Image	Note
TS20: basal columns	Present	Regionally restricted	3D	Expression is in the ventral horn.
TS20: tail sclerotome	Strong		3D
TS20: dorsal root ganglion	Present		3D
TS20: notochord	Present		3D

Structure	Level	Pattern	Image	Note
TS21: lens fiber	Present		4B
TS21: neural retinal epithelium	Present		4B

Structure	Level	Pattern	Image	Note
TS25: lens	Present		4C
TS25: retina outer nuclear layer	Present		4C

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present		4F	Expression is in the dental papilla and odontoblasts.
TS26: enamel organ	Present		4F

Structure	Level	Pattern	Image	Note
TS15: lung	Present		5A
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	5A	Expression is in the mesoderm contacting the gut.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	5B	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	5C	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: stomach	Present	Regionally restricted	5D	Expression is in the mucosal epithelium and smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS28: liver	Strong		5E

Structure	Level	Pattern	Image	Note
TS28: liver	Strong		5F

Structure	Level	Pattern	Image	Note
TS28: tongue	Present	Regionally restricted	5G	Expression is in the secretory cells.

Structure	Level	Pattern	Image	Note
TS28: tongue	Present	Regionally restricted	5H	Expression is in the secretory cells.

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	6A	Expression is in the endocardium and epicardium.

Structure	Level	Pattern	Image	Note
TS21: interventricular septum	Present		6C
TS21: heart ventricle	Present	Regionally restricted	6C	Expression is in the wall.

Structure	Level	Pattern	Image	Note
TS28: heart ventricle	Weak	Regionally restricted	6D	Expression is in the wall of the ventricle.
TS28: heart atrium	Absent		6D

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Regionally restricted	6F	Expression is in the glomerular capillaries.

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong		7A

Structure	Level	Pattern	Image	Note
TS25: axial musculature	Present		7B

Structure	Level	Pattern	Image	Note
TS23: rib	Present		7D

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	7F	Expression is in the surface ectoderm of the limb.

Structure	Level	Pattern	Image	Note
TS28: skin	Present		7G

Structure	Level	Pattern	Image	Note
TS28: skin	Present		7H