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Gene Expression Data
Assay Details
Assay
Reference: J:21860 Jin Y, et al., Cloning and expression of fibroblast growth factor receptor-1 isoforms in the mouse heart: evidence for isoform switching during heart development. J Mol Cell Cardiol. 1994 Nov;26(11):1449-59
Assay type: RT-PCR
MGI Accession ID: MGI:2683752
Gene symbol: Fgfr1
Gene name: fibroblast growth factor receptor 1
Probe: Fgfr1 P1, Fgfr1 P2
Visualized with: Ethidium bromide
Results Image: 6
 Sample Information Bands Other Sample Information
Lane AgeStructure 846 bp 579 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
a E15.0 (a) TS23: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 20 cycles.
b E15.0 (a) TS23: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 25 cycles.
c E15.0 (a) TS23: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 28 cycles.
d E15.0 (a) TS23: heart Present (b) Present (b) 1 µg; total RNA Swiss Webster Not Specified Amplified for 30 cycles.
e E15.0 (a) TS23: heart Present Present 1 µg; total RNA Swiss Webster Not Specified Amplified for 35 cycles.
f E15.0 (a) TS23: heart Present Present 1 µg; total RNA Swiss Webster Not Specified Amplified for 40 cycles.
g P adult TS28: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 20 cycles.
h P adult TS28: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 25 cycles.
i P adult TS28: heart Not Specified Not Specified 1 µg; total RNA Swiss Webster Not Specified Amplified for 28 cycles.
j P adult TS28: heart Present (c) Present (c) 1 µg; total RNA Swiss Webster Not Specified Amplified for 30 cycles.
k P adult TS28: heart Present Present 1 µg; total RNA Swiss Webster Not Specified Amplified for 35 cycles.
l P adult TS28: heart Present Present 1 µg; total RNA Swiss Webster Not Specified Amplified for 40 cycles.
m Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors state that the relative level of short versus long isoforms was 0.5 (67% long, 33% short) at 30 cycles.
(c) Authors state that the relative level of short versus long isoforms was 5.9 (15% long, 85% short) at 30 cycles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory