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Gene Expression Data
Assay Details
Assay
Reference: J:21860 Jin Y, et al., Cloning and expression of fibroblast growth factor receptor-1 isoforms in the mouse heart: evidence for isoform switching during heart development. J Mol Cell Cardiol. 1994 Nov;26(11):1449-59
Assay type: RT-PCR
MGI Accession ID: MGI:2683746
Gene symbol: Fgfr1
Gene name: fibroblast growth factor receptor 1
Probe: Fgfr1 P3, Fgfr1 P4
Visualized with: Ethidium bromide
Assay notes: Restriction enzyme digestion was used for detection of exon III splice variants.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure 786 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
a E15.0 (a) TS23: heart Present 1 µg; total RNA Swiss Webster Not Specified Uncut RT-PCR products.
b E15.0 (a) TS23: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with AccI.
c E15.0 (a) TS23: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with NaeI.
d E15.0 (a) TS23: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with HpaII.
e P adult TS28: heart Present 1 µg; total RNA Swiss Webster Not Specified Uncut RT-PCR products.
f P adult TS28: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with AccI.
g P adult TS28: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with NaeI.
h P adult TS28: heart Present 1 µg; total RNA Swiss Webster Not Specified Cut with HpaII.
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Some lanes contained digestion products; all were specific for exon IIIc.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory