Antibody:	anti-SYN/COR
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Specimens were fixed in 1% paraformaldehyde. Authors report that the antibodies used were a complex mixture that recognizes elements of the synaptonemal complex of meiotic chromosomes (Dobson et al., 1994, J. Cell Sci. 107, 2749-2760); Sycp1 and Sycp3 have been annotated.

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	4A 13.5 dpc lower , 4A 13.5 dpc upper	Expression in an anterior to posterior pattern; germ cells became positive at the anterior end first.
TS21: germ cell of ovary	Present		4A 13.5 dpc upper , 4A 13.5 dpc lower	Punctate pattern of staining in the nuclei of some germ cells.

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	4A 14.5 dpc upper , 4A 14.5 dpc lower	Expression in an anterior to posterior pattern; germ cells became positive at the anterior end first.
TS22: germ cell of ovary	Present		4A 14.5 dpc upper , 4A 14.5 dpc lower	Staining in germ cells showed a filamentous pattern.

Structure	Level	Pattern	Image	Note
TS23: ovary	Present	Regionally restricted	4A 15.5 dpc lower , 4A 15.5 dpc upper	Expression in germ cells throughout the gonad, in both cortex and medulla.
TS23: germ cell of ovary	Present		4A 15.5 dpc upper , 4A 15.5 dpc lower

Structure	Level	Pattern	Image	Note
TS21: testis	Ambiguous		5A 13.5 dpc
TS21: germ cell of testis	Absent		5A 13.5 dpc

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Regionally restricted	5C , 5A 14.5 dpc
TS22: germ cell of testis	Present		5A 14.5 dpc , 5C	Authors state that 20-30 positive germ cells were found in the anterior junction between the gonad and mesonephros. Positive cells were located both inside and outside testis cord structures and in the mesonephros.
TS22: mesonephros	Present	Regionally restricted	5C , 5A 14.5 dpc

Structure	Level	Pattern	Image	Note
TS23: testis	Ambiguous		5A 15.5 dpc
TS23: germ cell of testis	Absent		5A 15.5 dpc