Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:51694
Paynton BV, RNA-binding proteins in mouse oocytes and embryos: expression of genes encoding Y box, DEAD box RNA helicase, and polyA binding proteins. Dev Genet. 1998;23(4):285-98
|
|
Assay type:
|
RT-PCR
|
|
MGI Accession ID:
|
MGI:2682901
|
|
Gene symbol:
|
Pabpc1
|
|
Gene name:
|
poly(A) binding protein, cytoplasmic 1
|
|
Probe:
|
P3, P4
|
|
Assay notes:
|
These RT-PCR primers also amplify a related gene Pabpc2 (see assay MGI:2682904). The amplified products are the same size, so this assay does not differentiate between the 2 genes.
|
|
|
Results
|
Image: 9B
| P adult
|
TS28: oocyte
|
Not Specified
|
Not Specified; total RNA |
involves: BALB/cJ * C57BL/6J |
| Female |
50-200 growing oocytes.
|
| P adult
|
TS28: primary oocyte
|
Not Specified
|
Not Specified; total RNA |
involves: BALB/cJ * C57BL/6J |
| Female |
50-200 primary oocytes.
|
| P adult
|
TS28: secondary oocyte
|
Not Specified
|
Not Specified; total RNA |
involves: BALB/cJ * C57BL/6J |
| Female |
50-200 secondary oocytes.
|
| E2.0-2.5
(a)
|
TS3: 4-8 cell stage conceptus
|
Not Specified
|
Not Specified; total RNA |
involves: BALB/cJ * C57BL/6J |
| Pooled |
50-200 4-8 cell embryos.
|
| P adult
|
TS28: ovary
|
Not Specified
|
0.5-1.0 µg |
Not Specified |
| Female |
|
| Mol. Wt. Marker Lane |
Notes:
|
Analysis Tools