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Gene Expression Data
Assay Details
Assay
Reference: J:8621 Weydert A, et al., Developmental pattern of mouse skeletal myosin heavy chain gene transcripts in vivo and in vitro. Cell. 1987 Apr 10;49(1):121-9
Assay type: RNase protection
MGI Accession ID: MGI:2682561
Gene symbol: Myh3
Gene name: myosin, heavy polypeptide 3, skeletal muscle, embryonic
Probe: Myhse cDNA
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Quantification of these data is provided in Fig. 2.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure 350 bp 333 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 E14.5 (a) TS22: forelimb
TS22: hindlimb 		Present Present .225 µg; poly-A+ RNA C3H Not Specified
Lane 2 E17.5 (a) TS25: hindlimb musculature Present Present .105 µg; poly-A+ RNA C3H Not Specified
Lane 3 P1-2 TS27: hindlimb musculature Present Present .435 µg; total RNA C3H Not Specified
Lane 4 P5-6 TS28: hindlimb musculature Present Present .467 µg; total RNA C3H Not Specified
Lane 5 P11-12 TS28: hindlimb musculature Present Present .572 µg; total RNA C3H Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The amount of mRNA present decreases after birth.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory