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Gene Expression Data
Assay Details
Assay
Reference: J:38799 Mardon G, et al., The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers. Cell. 1989 Mar 10;56(5):765-70
Assay type: Northern blot
MGI Accession ID: MGI:2682552
Gene symbol: Zfy1
Gene name: zinc finger protein 1, Y-linked
Probe: pDP1055
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: This data was discussed in the text but was not shown. Under the stringent conditions used by the authors, the probe used in this assay hybridizes to transcripts from two genes. (At a stringency lower than that used in this assay, the probe hybridizes to a third gene.) Similar results were obtained using probes MGI:35783 and MGI:20248.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E12 female E12.0 (a) TS20: embryo Absent 5 µg; poly-A+ RNA FVB/N Female
E12 male E12.0 (a) TS20: embryo Absent 5 µg; poly-A+ RNA FVB/N Male
E14 female E14.0 (a) TS22: embryo Absent 5 µg; poly-A+ RNA FVB/N Female
E14 male E14.0 (a) TS22: embryo Absent 5 µg; poly-A+ RNA FVB/N Male
E16 female E16.0 (a) TS24: embryo Absent 5 µg; poly-A+ RNA FVB/N Female
E16 male E16.0 (a) TS24: embryo Absent 5 µg; poly-A+ RNA FVB/N Male
E18 female E18.0 (a) TS26: embryo Absent 5 µg; poly-A+ RNA FVB/N Female
E18 male E18.0 (a) TS26: embryo Absent 5 µg; poly-A+ RNA FVB/N Male
nb brain female P newborn TS27: brain Absent 5 µg; poly-A+ RNA FVB/N Female
nb brain male P newborn TS27: brain Absent 5 µg; poly-A+ RNA FVB/N Male
ad brain female P adult TS28: brain Absent 5 µg; poly-A+ RNA FVB/N Female
ad brain male P adult TS28: brain Absent 5 µg; poly-A+ RNA FVB/N Male
nb ovary P newborn TS27: ovary Absent 5 µg; poly-A+ RNA FVB/N Female
ad ovary P adult TS28: ovary Absent 5 µg; poly-A+ RNA FVB/N Female
nb heart female P newborn TS27: heart Absent 5 µg; poly-A+ RNA FVB/N Female
nb heart male P newborn TS27: heart Absent 5 µg; poly-A+ RNA FVB/N Male
ad heart female P adult TS28: heart Absent 5 µg; poly-A+ RNA FVB/N Female
ad heart male P adult TS28: heart Absent 5 µg; poly-A+ RNA FVB/N Male
nb kidney female P newborn TS27: metanephros Absent 5 µg; poly-A+ RNA FVB/N Female
nb kidney male P newborn TS27: metanephros Absent 5 µg; poly-A+ RNA FVB/N Male
ad kidney female P adult TS28: metanephros Absent 5 µg; poly-A+ RNA FVB/N Female
ad kidney male P adult TS28: metanephros Absent 5 µg; poly-A+ RNA FVB/N Male
nb lung female P newborn TS27: lung Absent 5 µg; poly-A+ RNA FVB/N Female
nb lung male P newborn TS27: lung Absent 5 µg; poly-A+ RNA FVB/N Male
ad lung female P adult TS28: lung Absent 5 µg; poly-A+ RNA FVB/N Female
ad lung male P adult TS28: lung Absent 5 µg; poly-A+ RNA FVB/N Male
nb spleen female P newborn TS27: spleen Absent 5 µg; poly-A+ RNA FVB/N Female
nb spleen male P newborn TS27: spleen Absent 5 µg; poly-A+ RNA FVB/N Male
ad spleen female P adult TS28: spleen Absent 5 µg; poly-A+ RNA FVB/N Female
ad spleen male P adult TS28: spleen Absent 5 µg; poly-A+ RNA FVB/N Male
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory