Mas1 RNase protection Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:74782 Alenina N, et al., Imprinting of the murine MAS protooncogene is restricted to its antisense RNA. Biochem Biophys Res Commun. 2002 Jan 25;290(3):1072-8
Assay type:	RNase protection
MGI Accession ID:	MGI:2682291
Gene symbol:	Mas1
Gene name:	MAS1 oncogene

Probe:	probe 3
Probe preparation:	Antisense RNA
Visualized with:	Phosphorimaging
Assay notes:	The probe was complementary to the antisense Mas1. The hybridization is not affected by the Neo insertion in Mas-deficient mice since the probe is located downstream of the Neo cassette.

Results

Image: 7

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	350 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	P m 3	TS28: skeletal muscle	Present	50 µg; total RNA	Not Specified		Not Specified
+/-	P m 3	TS28: skeletal muscle	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: skeletal muscle	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: skeletal muscle	Present (a)	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Not Specified
+/+	P m 3	TS28: uterus	Present	50 µg; total RNA	Not Specified		Female
+/-	P m 3	TS28: uterus	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Female	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: uterus	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Female	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: uterus	Present (a)	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Female
+/+	P m 3	TS28: hindbrain	Present	50 µg; total RNA	Not Specified		Not Specified
+/-	P m 3	TS28: hindbrain	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: hindbrain	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: hindbrain	Present (a)	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Not Specified
+/+	P m 3	TS28: placenta	Present	50 µg; total RNA	Not Specified		Female
+/-	P m 3	TS28: placenta	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Female	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: placenta	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Female	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: placenta	Present (a)	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Female
Y+	Control
Y-	Control

Notes:
(a) See assay note above.

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