Mas1 RNase protection Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:74782 Alenina N, et al., Imprinting of the murine MAS protooncogene is restricted to its antisense RNA. Biochem Biophys Res Commun. 2002 Jan 25;290(3):1072-8
Assay type:	RNase protection
MGI Accession ID:	MGI:2682287
Gene symbol:	Mas1
Gene name:	MAS1 oncogene

Probe:	Mas genomic3
Probe preparation:	Antisense RNA
Visualized with:	Phosphorimaging

Results

Image: 4

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	187 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	P m 3	TS28: metanephros	Present	50 µg; total RNA	Not Specified		Not Specified
+/-	P m 3	TS28: metanephros	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: metanephros	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: metanephros	Absent	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Not Specified
+/+	P m 3	TS28: testis	Present	50 µg; total RNA	Not Specified		Male
+/-	P m 3	TS28: testis	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Male	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: testis	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Male	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: testis	Absent	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Male
+/+	P m 3	TS28: heart	Present	50 µg; total RNA	Not Specified		Not Specified
+/-	P m 3	TS28: heart	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: heart	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: heart	Absent	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Not Specified
+/+	P m 3	TS28: forebrain	Present	50 µg; total RNA	Not Specified		Not Specified
+/-	P m 3	TS28: forebrain	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The wild-type allele is from the mother and the mutated allele is from the father.
-/+	P m 3	TS28: forebrain	Present	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1⁺	Not Specified	The mutated allele is from the mother and the wild-type allele is from the father.
-/-	P m 3	TS28: forebrain	Absent	50 µg; total RNA	involves: 129P2/OlaHsd	Mas1^tm1Bdr/Mas1^tm1Bdr	Not Specified
Y+	Control
Y-	Control

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