Pou4f1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:48044 Wyatt S, et al., NT-3 regulates expression of Brn3a but not Brn3b in developing mouse trigeminal sensory neurons. Brain Res Mol Brain Res. 1998 Apr;55(2):254-64
Assay type:	RT-PCR
MGI Accession ID:	MGI:2681907
Gene symbol:	Pou4f1
Gene name:	POU domain, class 4, transcription factor 1

Probe:	Brn3a-pA, Brn3a-pB
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The authors were unclear on which knock-out mouse were used in these studies. It is possible that Ntf3<tm1Jae> (MGI:1857232) was used. The probe used in this assay was 5' labelled.

Results

Image: 7_-/-

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
11	E11.0 (a)	TS18: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
12	E12.0 (a)	TS20: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
13	E13.0 (a)	TS21: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
14	E14.0 (a)	TS22: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
15	E15.0 (a)	TS23: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
16	E16.0 (a)	TS24: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
17	E17.0 (a)	TS25: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified
18	E18.0 (a)	TS26: trigeminal ganglion	Present	Not Specified; total RNA	involves: 129S2/SvPas	Ntf3^tm1Lfr/Ntf3^tm1Lfr	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There are 60% fewer trigeminal neurons in knock-out mice at birth compared to wild-type mice. However, in general there were similar amounts of mRNA per neuron in knock-out compared to mutant mice, except at E15 and E16 when the mRNA levels are reduced.

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