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Gene Expression Data
Assay Details
Assay
Reference: J:48044 Wyatt S, et al., NT-3 regulates expression of Brn3a but not Brn3b in developing mouse trigeminal sensory neurons. Brain Res Mol Brain Res. 1998 Apr;55(2):254-64
Assay type: RT-PCR
MGI Accession ID: MGI:2681907
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Probe: Brn3a-pA, Brn3a-pB
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The authors were unclear on which knock-out mouse were used in these studies. It is possible that Ntf3<tm1Jae> (MGI:1857232) was used. The probe used in this assay was 5' labelled.
Results Image: 7_-/-
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
11 E11.0 (a) TS18: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
12 E12.0 (a) TS20: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
13 E13.0 (a) TS21: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
14 E14.0 (a) TS22: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
15 E15.0 (a) TS23: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
16 E16.0 (a) TS24: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
17 E17.0 (a) TS25: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
18 E18.0 (a) TS26: trigeminal ganglion Present Not Specified; total RNA involves: 129S2/SvPas Ntf3tm1Lfr/Ntf3tm1LfrNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There are 60% fewer trigeminal neurons in knock-out mice at birth compared to wild-type mice. However, in general there were similar amounts of mRNA per neuron in knock-out compared to mutant mice, except at E15 and E16 when the mRNA levels are reduced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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