Nefm RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:33482 Pernas-Alonso R, et al., Early upregulation of medium neurofilament gene expression in developing spinal cord of the wobbler mouse mutant. Brain Res Mol Brain Res. 1996 Jun;38(2):267-75
Assay type:	RT-PCR
MGI Accession ID:	MGI:2681765
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Probe:	NFM-pA, NFM-pB
Visualized with:	Phosphorimaging

Results

Image: 4B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	471 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
C8	E18.0 (a)	TS26: spinal cord	Present (b)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54^wr	Not Specified	This animal's genotype is putative, not confirmed.
C9	E18.0 (a)	TS26: spinal cord	Present (b)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54^wr	Not Specified	This animal's genotype is putative, not confirmed.
C10	E18.0 (a)	TS26: spinal cord	Present (b)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54^wr	Not Specified	This animal's genotype is putative, not confirmed.
C1	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C2	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C3	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C5	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C6	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C7	E18.0 (a)	TS26: spinal cord	Present (c)	Not Specified	involves: C57BL/Fa * CD-1	Vps54^wr/Vps54⁺	Not Specified	This animal's genotype is putative, not confirmed.
C4	E18.0 (a)	TS26: spinal cord	Present	Not Specified	CD-1		Not Specified	This animal's genotype is putative, not confirmed.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This embryo's expression level is four times greater than that seen in the presumed wild type embryos.
(c) This embryo's expression level is intermediate between the highest expressing embryos and the presumed wild type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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