Summary | Mutation origin | Mutation description | Expression | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Bbc3tm1Ast
Name:	BCL2 binding component 3; targeted mutation 1, Andreas Strasser
MGI ID:	MGI:2681654
Synonyms:	puma-
Gene:	Bbc3  Location: Chr7:16042318-16052130 bp, + strand  Genetic Position: Chr7, 8.85 cM
Alliance:	Bbc3tm1Ast page

Germline Transmission:	Earliest citation of germline transmission: J:86466
Parent Cell Line:	Bruce 4 (ES Cell)
Strain of Origin:	B6.Cg-Thy1a

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exons 2 and 3 which contain the BH3 interaction domain were replaced by a floxed PGK-neomycin expression cassette. The resistance cassette was removed in vivo by crossing mice to a C57BL/6 Deleter mouse line. Gene disruption was confirmed by RT-PCR and Northern blot analysis. (J:86466)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

List all tumor models in MMHCdb carrying Bbc3tm1Ast

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bbc3 Mutation:	18 strains or lines available

Original:	J:86466 Villunger A, et al., p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science. 2003 Nov 7;302(5647):1036-8
All:	70 reference(s)