Mapt Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83370 Takuma H, et al., Isoforms changes of tau protein during development in various species. Brain Res Dev Brain Res. 2003 May 14;142(2):121-7
Assay type:	Western blot
MGI Accession ID:	MGI:2681601
Gene symbol:	Mapt
Gene name:	microtubule-associated protein tau

Antibody:	Pool-2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The proteins used for this assay were dephophorylated. The antibody used in this assay is thought to recognize all isoforms. There is an additional band seen on the gel (labeled c'); it is thought to be degraded or a partially dephosphorylated form of the protein.

Results

Image: 3A

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (d)	Size Not Specified (e)	Genetic Background	Mutant Allele(s)	Sex
E14	E14.0 (a)	TS22: brain	Absent	Absent	Absent	Absent	ICR		Not Specified
E17	E17.0 (a)	TS25: brain	Present	Absent	Absent	Absent	ICR		Not Specified
P0	P0	TS27: brain	Not Specified	Absent	Absent	Absent	ICR		Not Specified
P14	P14	TS28: brain	Not Specified	Present	Absent	Absent	ICR		Not Specified
P28	P28	TS28: brain	Not Specified	Present	Present	Present	ICR		Not Specified
Adult	P adult	TS28: brain	Absent	Present	Present	Present	ICR		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band is thought to represent the no amino terminal insert, three repeat isoform. Its expression level decreases as development proceeds.
(c) This band is thought to represent the no amino terminal insert, four repeat isoform.
(d) This band is thought to represent the one amino terminal insert, four repeat isoform.
(e) This band is thought to represent the two amino terminal insert, four repeat isoform.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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