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Gene Expression Data
Assay Details
Assay
Reference: J:45221 Ozawa K, et al., A quantitative method for evaluation of FGF family and FGF receptor family gene expression by RT-PCR. Brain Res Brain Res Protoc. 1997 Aug;1(3):211-6
Assay type: RT-PCR
MGI Accession ID: MGI:2681516
Gene symbol: Fgf1
Gene name: fibroblast growth factor 1
Probe: Fgf1-pA, Fgf1-pB
Probe preparation: Double stranded labelled with digoxigenin
Visualized with: Autoradiography
Assay notes: Identity of the RT-products was verified by hybridization with a gene specific probe.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure 387 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 E12.0 (a) TS20: brain Present 1 µg; total RNA ICR Not Specified The figure legend labels this lane P12, but that age is inconsistent with the rest of the figure.
Lane 2 E14.0 (a) TS22: brain Present 1 µg; total RNA ICR Not Specified
Lane 3 E16.0 (a) TS24: brain Present 1 µg; total RNA ICR Not Specified
Lane 4 P1 TS27: cerebellum Present 1 µg; total RNA ICR Not Specified
Lane 5 P3 TS27: brain Present 1 µg; total RNA ICR Not Specified
Lane 6 P5 TS28: brain Present 1 µg; total RNA ICR Not Specified
Lane 7 P adult TS28: cerebellum Present 1 µg; total RNA ICR Not Specified
Lane 8 P adult TS28: cerebellum Present 1 µg; total RNA ICR Not Specified
Lane 9 Control
Lane 10 Control
Lane 11 Control
Lane 12 Control
Lane 13 Control
Lane 14 Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Quantification is provided in the lower portion of the figures; expression levels increased as development progressed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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