Probe:	Slitrk1 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present		3a
TS25: thalamus	Present		3a
TS25: hypothalamus	Present	Regionally restricted	3a	Expression was detected in the ventromedial hypothalamus.
TS25: midbrain	Present	Regionally restricted	3a	Expression was detected in the medial part of the periaqueductal gray matter.
TS25: hippocampus	Present	Regionally restricted	3a	Expression was detected in the pyramidal layer.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	3g	Expression was detected in the subventricular zone, the subplate and the deeper layer of the cortical plate.
TS25: cerebral cortex subventricular zone	Present		3g
TS25: cortical plate	Present	Regionally restricted	3g	Expression was detected in the subventricular zone, the subplate and the deeper layer of the cortical plate.

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Present		4a

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present		4c

Structure	Level	Pattern	Image	Note
TS25: spinal cord mantle layer	Present		4e
TS25: basal columns	Present	Regionally restricted	4e	Expression was detected in the lateral and medial motor columns.
TS25: spinal cord ventricular layer	Absent		4e	Expression was absent from the ventricular neural cells.