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Gene Expression Data
Assay Details
Assay
Reference: J:50181 Wu L, et al., Differential expression of Ped gene candidates in preimplantation mouse embryos. Biol Reprod. 1998 Oct;59(4):941-52
Assay type: RT-PCR
MGI Accession ID: MGI:2679966
Gene symbol: H2-Q7
Gene name: histocompatibility 2, Q region locus 7
Probe: Q7/Q9-pA, Q7/Q9-pB
Visualized with: Ethidium bromide
Assay notes: This primer set amplified two closely related genes. SNuPE was needed to distinguish between them.
Results Image: 3_lanes 4-6
 Sample Information Bands Other Sample Information
Lane AgeStructure 380 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
MW Mol. Wt. Marker Lane
Q6/Q8 1 Lane not used in this assay
Q6/Q8 2 Lane not used in this assay
Q6/Q8 3 Lane not used in this assay
Q7/Q9 4 P TS28: spleen Absent Not Specified CBA/Ca Not Specified Splenic lymphocytes were isolated using a Ficoll-Hypaque density gradient.
Q7/Q9 5 P TS28: spleen Present Not Specified C57BL/6 Not Specified Splenic lymphocytes were isolated using a Ficoll-Hypaque density gradient.
Q7/Q9 6 P TS28: spleen Absent (a) Not Specified B6.K2 Not Specified Splenic lymphocytes were isolated using a Ficoll-Hypaque density gradient.
Notes:
(a) SNuPE analysis done elsewhere in the paper showed the only the Q9 gene, not the Q7 one, was expressed in lymphocytes of B6.K2 mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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