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Gene Expression Data
Assay Details
Assay
Reference: J:68198 Robertson SA, et al., Granulocyte-macrophage colony-stimulating factor promotes glucose transport and blastomere viability in murine preimplantation embryos. Biol Reprod. 2001 Apr;64(4):1206-15
Assay type: RT-PCR
MGI Accession ID: MGI:2679225
Gene symbol: Csf2rb
Gene name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Probe: Bc-pC, Bc-pD
Visualized with: Ethidium bromide
Assay notes: Nested primer sets were used for this assay; the outer pair is MGI:2679219. Authors also used a more sensitive procedure--using P32 dATP in the reaction mix, electrophoresing the products, transferring the products to nylon, and subjecting the blot to autoradiography. This procedure also failed to detect expression in the embryo.
Results Image: 2A B[c]
 Sample Information Bands Other Sample Information
Lane AgeStructure 180 bp Amount Genetic BackgroundMutant Allele(s)Sex
MW Mol. Wt. Marker Lane
1-cell E0.5 (a) TS1: 1-cell stage conceptus Absent Not Specified; total RNA (BALB/c x CBA)F1 Not Specified
2-cell E1.0 (a) TS2: 2-cell stage conceptus Absent Not Specified; total RNA (BALB/c x CBA)F1 Not Specified
8-cell E2.5 (a) TS3: 8-cell stage conceptus Absent Not Specified; total RNA (BALB/c x CBA)F1 Not Specified
blast E3.5 (a) TS4: blastocyst-stage conceptus Absent Not Specified; total RNA (BALB/c x CBA)F1 Not Specified
FD5/12 In vitro data: no data stored
mUt P TS28: uterus Present Not Specified; total RNA Not Specified Female
Lane 8 Control
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory