Enah Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:3109 Sazuka T, et al., Identification of a developmentally regulated gene in the mouse central nervous system which encodes a novel proline rich protein. Biochim Biophys Acta. 1992 Oct 20;1132(3):240-8
Assay type:	Northern blot
MGI Accession ID:	MGI:2678782
Gene symbol:	Enah
Gene name:	ENAH actin regulator

Probe:	NDPP-1 cDNA
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging

Results

Image: 3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E10	E10.0 (a)	TS16: head	Weak (c)	5 µg; poly-A+ RNA	Not Specified		Not Specified
E11	E11.0 (a)	TS18: head	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
E13	E13.0 (a)	TS21: brain	Weak (d)	5 µg; poly-A+ RNA	Not Specified		Not Specified
E15	E15.0 (a)	TS23: brain	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
E17	E17.0 (a)	TS25: brain	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
N1	P1	TS27: brain	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
N5	P5	TS28: brain	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
ADULT	P adult	TS28: brain	Weak	5 µg; poly-A+ RNA	Not Specified		Not Specified
PC12(-)	In vitro data: no data stored
PC12+NGF(2d)	In vitro data: no data stored
PC12+NGF(4d)	In vitro data: no data stored
PC12+NGF(6d)	In vitro data: no data stored
PCC4(-)	In vitro data: no data stored
PCC4+RA	In vitro data: no data stored

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The steady state levels of all mRNA species were observed to be developmentally down-regulated, so that they were only faintly detectable in the adult brain.
(c) There is a major species of 4.3 kb and a weaker one of 3.8 kb seen at this age, as well as a band at 2.3 kb.
(d) There is a prominent band at 5.4 kb at this age, as well as the major species of 4.3 kb and the weaker one of 3.8 kb seen earlier.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23