Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84598
Molyneaux KA, et al., GP130, the shared receptor for the LIF/IL6 cytokine family in the mouse, is not required for early germ cell differentiation, but is required cell-autonomously in oocytes for ovulation. Development. 2003 Sep;130(18):4287-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2677904
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Gene symbol:
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Il6st
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Gene name:
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interleukin 6 signal transducer
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Antibody:
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Anti-GP130
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Detection system:
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Secondary antibody coupled to Cy5
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Results
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Specimen
2B:
postnatal adult
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Weak |
Uniform |
2B
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Expression was weak and diffuse in primordial stage oocytes.
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| TS28: ovary |
Present |
Regionally restricted |
2B
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Expression was present in the thecal and granulosa cells of the follicle.
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Specimen
2C:
postnatal adult
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ovary |
Present |
Regionally restricted |
2C
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Expression was present in the thecal and granulosa cells of the follicle.
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| TS28: oocyte |
Present |
Regionally restricted |
2C
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Expression was detected on the cell surface of the growing oocyte.
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Specimen
2E:
postnatal adult
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary spermatocyte |
Present |
Regionally restricted |
2E
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Expression was present on the cell surface of primary spermatocytes in seminiferous tubules exhibiting a few elongating spermatids.
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Specimen
2F:
postnatal adult
(more )
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatid |
Present |
Regionally restricted |
2F
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Expression was present on the surface of all spermatocytes and spermatids in seminiferous tubules containing mature sperm.
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| TS28: spermatocyte |
Present |
Regionally restricted |
2F
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Expression was present on the surface of all spermatocytes and spermatids in seminiferous tubules containing mature sperm.
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Specimen
4C:
postnatal adult
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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4C
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Expression was detected in an antral stage oocyte.
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Analysis Tools