Probe:	pIrx5a
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Authors described two probes and said that similar expression patterns were detected with each; it is possible MGI:2677432 was used to generate some or all of these data.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2C	Expression is throughout the dorsolateral region, absent at the mid/hindbrain boundary.
TS15: future hindbrain	Present	Regionally restricted	2C	Expression is in the ventrolateral region of the metencephalon, absent at the mid/hindbrain boundary.
TS15: neural tube lateral wall	Present	Regionally restricted	2C	Expression is in the ventricular layer.
TS15: forelimb bud	Present		2C
TS15: hindlimb ridge	Present		2C
TS15: heart atrium	Present		2C

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	2H	Expression is in the ventricular layer.
TS17: forelimb bud	Present		2H
TS17: primitive ventricle	Present		2H
TS17: heart atrium	Present		2H
TS17: branchial arch	Present		2H
TS17: foregut	Present		2H

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present	Regionally restricted	3A	Expression is in motor neurons.

Structure	Level	Pattern	Image	Note
TS21: basal columns	Present	Regionally restricted	3B	Expression is in motor neurons.
TS21: spinal cord marginal layer	Present		3B
TS21: spinal cord ventricular layer	Present		3B

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		4I

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		4J