Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85474
Cheng HT, et al., {gamma}-Secretase activity is dispensable for mesenchyme-to-epithelium transition but required for podocyte and proximal tubule formation in developing mouse kidney. Development. 2003 Oct;130(20):5031-5042
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2676303
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Gene symbol:
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Notch1
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Gene name:
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notch 1
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Antibody:
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anti-cleaved Notch1 (Val1744) antibody (anti-NICD) (#2421)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Incubation with primary antibody was followed by biotinylated anti-rabbit IgG, avidin/biotinylated HRP complex, amplification reagent biotinyl-tyramide, streptavidin-HRP, then DAB.
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Results
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Specimen
1A:
embryonic day 14.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metanephros |
Present |
Regionally restricted |
1A
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Expression in the nucleus of S- and comma-shaped bodies.
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Analysis Tools
