Acan RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:41498 Watanabe H, et al., Dwarfism and age-associated spinal degeneration of heterozygote cmd mice defective in aggrecan. Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6943-7
Assay type:	RT-PCR
MGI Accession ID:	MGI:2675627
Gene symbol:	Acan
Gene name:	aggrecan

Probe:	aggrecan-pA, aggrecan-pB
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The authors performed a quantitative RT-PCR analysis using a constant amount of cDNA and of the two oligonucleotide primers specific for the gene of interest, while varying the amount of neutral mimic DNA used as a competitor. Those blots and graphs arepictured in Fig. 2. This annotation is a summary of that data.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
wild type	E15.0 (a)	TS23: limb cartilage condensation	Present	.2 µg; poly-A+ RNA	Not Specified		Not Specified
heterozygote	E15.0 (a)	TS23: limb cartilage condensation	Present (b)	.2 µg; poly-A+ RNA	involves: STOCK T t^low Itpr3^tf	Acan^cmd/Acan⁺	Not Specified
homozygote	E15.0 (a)	TS23: limb cartilage condensation	Present (c)	.2 µg; poly-A+ RNA	involves: STOCK T t^low Itpr3^tf	Acan^cmd/Acan^cmd	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are at 81 percent of that found in wild-type embryos.
(c) Expression levels are at 41 percent of that found in wild-type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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