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Gene Expression Data
Assay Details
Assay
Reference: J:28981 Fiering S, et al., Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes Dev. 1995 Sep 15;9(18):2203-13
Assay type: RT-PCR
MGI Accession ID: MGI:2673933
Gene symbol: Hbb-bs
Gene name: hemoglobin, beta adult s chain
Probe: beta-m-4, beta-m-3R
Probe preparation: DNA
Visualized with: Autoradiography
Assay notes: RT reaction was performed on RNA extracted from single (C57BL6/J) and diffuse (129Sv) haplotype mice. Assays were done by mixing the RT reactions from mice of each haplotype in denoted amounts and amplifying by PCR. PCR products were subsequently digested with BstXI, then with PvuI.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 200 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex
E15,5 fetal liver, wt E15.5 (a) TS23: liver Present Not Specified involves: 129 * C57BL/6J Not Specified
E15.5 fetal liver, wt E15.5 (a) TS23: liver Present Not Specified involves: 129 * C57BL/6J Not Specified
E15.5 fetal liver, delta 2 E15.5 (a) TS23: liver Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E15.5 fetal liver, delta 2 E15.5 (a) TS23: liver Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E17.5 fetal liver, wt E17.5 (a) TS25: liver Present Not Specified involves: 129 * C57BL/6J Not Specified
E17.5 fetal liver, wt E17.5 (a) TS25: liver Present Not Specified involves: 129 * C57BL/6J Not Specified
E17.5 fetal liver, delta 2 E17.5 (a) TS25: liver Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E17.5 fetal liver, delta 2 E17.5 (a) TS25: liver Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
adult blood, wt P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
adult blood, wt P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
adult blood, delta 2 P adult TS28: blood Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
adult blood, delta 2 P adult TS28: blood Present Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) labeled "single"
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory