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Gene Expression Data
Assay Details
Assay
Reference: J:28981 Fiering S, et al., Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes Dev. 1995 Sep 15;9(18):2203-13
Assay type: RT-PCR
MGI Accession ID: MGI:2673932
Gene symbol: Hbb-bh1
Gene name: hemoglobin Z, beta-like embryonic chain
Probe: beta-h1-5F, beta-h1-5R
Probe preparation: DNA
Visualized with: Autoradiography
Assay notes: RT reaction was performed on RNA extracted from single (C57BL6/J) and diffuse (129Sv) haplotype mice. Assays were done by mixing the RT reactions from mice of each haplotype in denoted amounts, amplifying by PCR, and digesting with XbaI.
Results
Image: 5B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 270 Da (b) 270 Da (c) Amount Genetic BackgroundMutant Allele(s)Sex
E9,5 yolk sac, wt E9.5 (a) TS15: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E9.5 yolk sac, wt E9.5 (a) TS15: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E9.5 yolk sac, delta 2 E9.5 (a) TS15: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E9.5 yolk sac, delta 2 E9.5 (a) TS15: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E10.5 yolk sac, wt E10.5 (a) TS17: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E10.5 yolk sac, wt E10.5 (a) TS17: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E10.5 yolk sac, delta 2 E10.5 (a) TS17: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E10.5 yolk sac, delta 2 E10.5 (a) TS17: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E11.5 yolk sac, wt E11.5 (a) TS19: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E11.5 yolk sac, wt E11.5 (a) TS19: yolk sac Present Present Not Specified involves: 129 * C57BL/6J Not Specified
E11.5 yolk sac, delta 2 E11.5 (a) TS19: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
E11.5 yolk sac, delta 2 E11.5 (a) TS19: yolk sac Present Present (d) Not Specified involves: 129S4/SvJaeSor Rr320tm2Mkg/Rr320+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) labeled "single"
(c) labeled "diffuse"
(d) Authors report a two- to 4-fold reduction in expression of all [b]-like globin genes from the mutated chromosome.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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