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Gene Expression Data
Assay Details
Assay
Reference: J:28981 Fiering S, et al., Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes Dev. 1995 Sep 15;9(18):2203-13
Assay type: RT-PCR
MGI Accession ID: MGI:2673924
Gene symbol: Hbb-bs
Gene name: hemoglobin, beta adult s chain
Probe: beta-m-4, beta-m-3R
Probe preparation: DNA
Visualized with: Autoradiography
Assay notes: RT reaction was performed on RNA extracted from single (C57BL6/J) and diffuse (129Sv) haplotype mice. Assays were done by mixing the RT reactions from mice of each haplotype in denoted amounts and amplifying by PCR. PCR products were subsequently digested with BstXI, then with PvuI.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 200 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
100/0 P adult TS28: blood Present Not Specified C57BL/6J Not Specified
90/10 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
80/20 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
70/30 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
60/40 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
50/50 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
40/60 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
30/70 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
20/80 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
10/90 P adult TS28: blood Present Not Specified involves: 129 * C57BL/6J Not Specified
0/100 P adult TS28: blood Absent Not Specified 129/Sv Not Specified
Notes:
(a) labeled "single"

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory