Wnt5a RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70601 Lickert H, et al., Expression patterns of Wnt genes in mouse gut development. Mech Dev. 2001 Jul;105(1-2):181-4
Assay type:	RNA in situ
MGI Accession ID:	MGI:2673731
Gene symbol:	Wnt5a
Gene name:	wingless-type MMTV integration site family, member 5A

Probe:	Wnt5a cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen 1--E12.5 WM: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	1_Wnt5a E12.5	Expression is in the entire fundus and part of the corpus region.
TS20: gut	Strong	Regionally restricted	1_Wnt5a E12.5	Expression is in the sphincter region which separates the future small intestine and bowel. Absent in the appendix.
TS20: foregut duodenum	Weak		1_Wnt5a E12.5
TS20: midgut derived duodenum	Weak		1_Wnt5a E12.5
TS20: hindgut	Absent		1_Wnt5a E12.5	Expression is absent in the most posterior region.

Specimen 1--E12.5 sxn: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Present	Uniform	2A Wnt5a E12.5 stom.

Specimen 1--E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: stomach fundus	Present		1_Wnt5a E14.5
TS22: stomach	Present	Regionally restricted	1_Wnt5a E14.5	Expression is in part of the corpus region.
TS22: gut	Present	Regionally restricted	1_Wnt5a E14.5	Expression is in the sphincter region which separates the future small intestine and bowel. Absent in the appendix
TS22: foregut duodenum	Weak		1_Wnt5a E14.5
TS22: midgut derived duodenum	Weak		1_Wnt5a E14.5
TS22: rectum	Absent		1_Wnt5a E14.5

Specimen 2A--E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: stomach mesenchyme	Present	Regionally restricted	2A Wnt5a E14.5 stom.	Expression in the mesenchyme surrounding the epithelium.

Specimen 2A--E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: duodenum	Present	Regionally restricted	2A Wnt5a E16.5 stom.	Expression in clumps of mesenchymal cells close to the epithelium including an additional ring of peripheral mesenchymal cells.
TS24: ileum	Present	Regionally restricted	2A Wnt5a E16.5 stom.	Expression in clumps of mesenchymal cells close to the epithelium including an additional ring of peripheral mesenchymal cells.
TS24: jejunum	Present	Regionally restricted	2A Wnt5a E16.5 stom.	Expression in clumps of mesenchymal cells close to the epithelium including an additional ring of peripheral mesenchymal cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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