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Gene Expression Data
Assay Details
Assay
Reference: J:82459 Laclef C, et al., Altered myogenesis in Six1-deficient mice. Development. 2003 May;130(10):2239-52
Assay type: RNA in situ
MGI Accession ID: MGI:2673286
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Probe: MyoD probe3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Identical results were obtained with comparison of the mutation on the 129/Sv and C57BL/6 strain backgrounds.
Results
Specimen 7A: embryonic day 11.5; Six1tm1Mair/Six1tm1Mair (more )
Structure Level Pattern Image Note
TS19: forelimb bud Absent 7A -/-
TS19: hindlimb bud Absent 7A -/-
Specimen 7B: embryonic day 11.5; Six1tm1Mair/Six1+ (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 7B +/-
TS19: hindlimb bud Present 7B +/-
Specimen 7C: embryonic day 11.5; Six1tm1Mair/Six1tm1Mair (more )
Structure Level Pattern Image Note
TS19: limb Absent 7C -/-
TS19: trunk dermomyotome Present Regionally restricted 7C -/- Compared to the heterozygous embryo, expression was reduced or absent in the epaxial most domain.
Specimen 7D: embryonic day 11.5; Six1tm1Mair/Six1+ (more )
Structure Level Pattern Image Note
TS19: limb Present 7D +/-
TS19: trunk dermomyotome Present 7D +/-
Specimen 7I: embryonic day 12.5; Six1tm1Mair/Six1tm1Mair (more )
Structure Level Pattern Image Note
TS20: forelimb Present Single cells 7I -/- Expression was detected in only a few cells in the forelimb shoulder of the mutant.
Specimen 7J: embryonic day 12.5; Six1tm1Mair/Six1+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 7J +/-
Specimen 7K +/-: embryonic day 12.5; Six1tm1Mair/Six1+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 7K +/-
Specimen 7K -/-: embryonic day 12.5; Six1tm1Mair/Six1tm1Mair (more )
Structure Level Pattern Image Note
TS20: forelimb Present Single cells 7K -/- Expression was detected in only a few cells compared to the heterozygous embryo. The forelimb was more affected than the hindlimb in the mutant.
Specimen 7L +/-: embryonic day 12.5; Six1tm1Mair/Six1+ (more )
Structure Level Pattern Image Note
TS20: hindlimb Present 7L +/-
Specimen 7L -/-: embryonic day 12.5; Six1tm1Mair/Six1tm1Mair (more )
Structure Level Pattern Image Note
TS20: hindlimb Present Regionally restricted 7L -/- Expression was detected in the ventral (a few cells) and dorsal (many cells) regions. The hindlimb was less affected than the forelimb in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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