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Gene Expression Data
Assay Details
Assay
Reference: J:47810 Karolczak M, et al., Developmental expression and regulation of aromatase- and 5alpha-reductase type I mRNA in the male and female mouse hypothalamus. J Neuroendocrinol. 1998 Apr;10(4):267-74
Assay type: RT-PCR
MGI Accession ID: MGI:2672873
Gene symbol: Srd5a1
Gene name: steroid 5 alpha-reductase 1
Probe: 5a-reductase-pA, 5a-reductase-pB
Visualized with: Ethidium bromide
Assay notes: The lane numbers indicate the number of PCR amplification cycles. Authors state that linear amplification was observed between 28 and 38 cycles. In Figure 2B, the RT-PCR gel was Southern blotted and hybridized with a DIG-labelled internal oligonucleotide probe (MGI:2672869) to demonstrate primer specificity.
Results Image: 2A
 Sample Information Bands Other Sample Information
Lane AgeStructure 419 bp Amount Genetic BackgroundMutant Allele(s)Sex
MW Mol. Wt. Marker Lane
28 P newborn TS27: hypothalamus Not Specified 10 µg; total RNA BALB/c Male
30 P newborn TS27: hypothalamus Not Specified 10 µg; total RNA BALB/c Male
32 P newborn TS27: hypothalamus Present 10 µg; total RNA BALB/c Male
34 P newborn TS27: hypothalamus Present 10 µg; total RNA BALB/c Male
38 P newborn TS27: hypothalamus Present 10 µg; total RNA BALB/c Male
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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