Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47810
Karolczak M, et al., Developmental expression and regulation of aromatase- and 5alpha-reductase type I mRNA in the male and female mouse hypothalamus. J Neuroendocrinol. 1998 Apr;10(4):267-74
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2672872
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Gene symbol:
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Cyp19a1
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Gene name:
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cytochrome P450, family 19, subfamily a, polypeptide 1
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Probe:
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aromatase-pA, aromatase-pB
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Visualized with:
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Ethidium bromide
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Assay notes:
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The lane numbers indicate the number of PCR amplification cycles. Authors state that linear amplification was observed between 28 and 38 cycles. In Figure 1B, the RT-PCR gel was Southern blotted and hybridized with a DIG-labelled internal oligonucleotide probe (MGI:2672867) to demonstrate primer specificity.
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Results
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Image: 1A
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Analysis Tools
