Ache RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:34235 Luo ZD, et al., Acetylcholinesterase and nicotinic acetylcholine receptor expression diverge in muscular dysgenic mice lacking the L-type calcium channel. J Neurochem. 1996 Jul;67(1):111-8
Assay type:	RNase protection
MGI Accession ID:	MGI:2672122
Gene symbol:	Ache
Gene name:	acetylcholinesterase

Probe:	AChE cDNA3
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography

Results

Image: 1_AChE CAM

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	456 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
NB +/+	P newborn	TS27: heart	Present	Not Specified; total RNA	Not Specified		Pooled
NB mdg/mdg	P newborn	TS27: heart	Present	Not Specified; total RNA	involves: 129P1/ReJ * STOCK T/t⁴	Cacna1s^mdg/Cacna1s^mdg	Not Specified
E18 +/+	E18.0 (b)	TS26: heart	Present	Not Specified; total RNA	Not Specified		Pooled
E18 mdg/mdg	E18.0 (b)	TS26: heart	Present	Not Specified; total RNA	involves: 129P1/ReJ * STOCK T/t⁴	Cacna1s^mdg/Cacna1s^mdg	Pooled

Notes:
(a) In mutants, the expression levels increased compared to controls. Expression levels are higher in skeletal muscle than in heart (see assay MGI:2672120).
(b) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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