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Gene Expression Data
Assay Details
Assay
Reference: J:79580 Le Bras S, et al., Transcript map of the Ovum mutant (Om) locus: isolation by exon trapping of new candidate genes for the DDK syndrome. Gene. 2002 Aug 21;296(1-2):75
Assay type: RT-PCR
MGI Accession ID: MGI:2671546
Gene symbol: Lig3
Gene name: ligase III, DNA, ATP-dependent
Probe: DNA Ligase III-pA, DNA LigaseIII-pB
Probe preparation: DNA
Assay notes: Two rounds of nested PCR was performed, the first round with primer set DNA Ligase III-pC, -pD (MGI:2671194), the second round with primer set DNA Ligase III-pA, -pB (MGI:2671193).
Results Image: 4_DNA ligaseIII
 Sample Information Bands Other Sample Information
Lane AgeStructure 1450 bp Amount Genetic BackgroundMutant Allele(s)Sex
DDK oocytes -RT Control
DDK oocytes +RT P adult TS28: oocyte Present 0.05 µg; total RNA DDK Female
non-DDK oocytes +RT P adult TS28: oocyte Present 0.05 µg; total RNA (C57BL/6 x SJL)F1 Female
water Control
DNA Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory