Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81324
Smart N, et al., A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Mech Dev. 2002 Dec;119 Suppl 1:S65-71
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2671345
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Gene symbol:
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Cst3
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Gene name:
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cystatin C
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Probe:
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Cst3 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 10.0
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: primitive ventricle |
Present |
Regionally restricted |
3A
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Most notable expression is in the outer curvature.
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Specimen
3B:
embryonic day 10.0
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: primitive ventricle |
Present |
Regionally restricted |
3B
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Most notable expression is in the outer curvature.
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Specimen
3C:
embryonic day 10.0
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3D:
embryonic day 10.0
(more )
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: primitive ventricle |
Present |
Regionally restricted |
3D
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Expression is in the outer curvature in more caudal regions of the left ventricle.
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Specimen
5C, +/+:
embryonic day 8.0
(more )
Specimen
5C, +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D, -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hand1tm1Jcc/Hand1tm1Jcc
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown, +/+:
embryonic day 8.0
(more )
Specimen
Not Shown, +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown, -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hand1tm1Jcc/Hand1tm1Jcc
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
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Expression in this mutant is comparable to wild-type embryos.
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Analysis Tools